Goggin Rachel, Jardeleza Camille, Wormald Peter-John, Vreugde Sarah
Department of Surgery-Otorhinolaryngology Head and Neck Surgery, University of Adelaide, Adelaide, SA, Australia.
Int Forum Allergy Rhinol. 2014 Mar;4(3):171-5. doi: 10.1002/alr.21259. Epub 2014 Jan 15.
Colloidal silver is an alternative medicine consisting of silver particles suspended in water. After using this solution as a nasal spray, the symptoms of a previously recalcitrant Staphylococcus aureus (S. aureus)-infected chronic rhinosinusitis patient were observed to have improved markedly. The aim of this study was to determine whether colloidal silver has any direct bactericidal effects on these biofilms in vitro.
S. aureus biofilms were grown from the ATCC 25923 reference strain on Minimum Biofilm Eradication Concentration (MBEC) device pegs, and treated with colloidal silver. Concentrations tested ranged from 10 to 150 μL colloidal silver diluted to 200 μL with sterile water in 50 μL cerebrospinal fluid (CSF) broth. Control pegs were exposed to equivalent volumes of CSF broth and sterile water. The sample size was 4 biomass values per treatment or control group. Confocal scanning laser microscopy and COMSTAT software were used to quantify biofilms 24 hours after treatment.
Significant differences from control were found for all concentrations tested bar the lowest of 10 μL colloidal silver in 200 μL. At 20 μL colloidal silver, the reduction in biomass was 98.9% (mean difference between control and treatment = -4.0317 μm(3) /μm(2) , p < 0.0001). A maximum biomass reduction of 99.8% was reached at both 100 and 150 μL colloidal silver (mean differences = -4.0681 and -4.0675μm(3) /μm(2) , respectively, p < 0.0001).
Colloidal silver directly attenuates in vitro S. aureus biofilms.
胶体银是一种替代药物，由悬浮在水中的银颗粒组成。在将该溶液用作鼻腔喷雾剂后，观察到一名先前难治的金黄色葡萄球菌（金葡菌）感染的慢性鼻窦炎患者的症状明显改善。本研究的目的是确定胶体银在体外对这些生物膜是否具有直接杀菌作用。
从美国典型培养物保藏中心（ATCC）25923参考菌株在最低生物膜根除浓度（MBEC）装置柱上培养金葡菌生物膜，并用胶体银处理。测试的浓度范围为10至150μL胶体银，用无菌水在50μL脑脊液（CSF）肉汤中稀释至200μL。对照柱暴露于等量的CSF肉汤和无菌水中。每个处理或对照组的样本量为4个生物量值。在处理24小时后，使用共聚焦扫描激光显微镜和COMSTAT软件对生物膜进行定量。
除了200μL中最低的10μL胶体银外，所有测试浓度与对照相比均有显著差异。在20μL胶体银时，生物量减少了98.9%（对照与处理之间的平均差异=-4.0317μm³/μm²，p<0.0001）。在100和150μL胶体银时，生物量最大减少了99.8%（平均差异分别为-4.0681和-4.0675μm³/μm²，p<0.0001）。
胶体银可直接减弱体外金葡菌生物膜。
