Fox G Q
Abteilung Neurochemie, Max-Planck-Institut für biophysikalische Chemie, Göttingen, Federal Republic of Germany.
Cell Tissue Res. 1987 Oct;250(1):115-23. doi: 10.1007/BF00214662.
The electric organs of embryonic Torpedo marmorata have been reacted with three cationic stains to evaluate the appearance and distribution of anionic sites. Ruthenium red, alcian blue and lysozyme were used at different pHs and found to react in a time-related manner to anionic components within the interelectrocyte space. The basal lamina covering the ventral electrocyte surface possesses the greatest number of anionic sites whereas growth cone, presynaptic terminal and glial membranes displayed almost no staining. Since this lamina serves as the exclusive substrate for ingrowing neurites during synaptogenesis, the results are consistent with the idea that charge distribution on the membrane surface may provide a necessary cue for neurite motility, extension and eventual synaptogenesis.
用三种阳离子染料对胚胎期的电鳐（Torpedo marmorata）的电器官进行反应，以评估阴离子位点的外观和分布。在不同pH值下使用钌红、阿尔辛蓝和溶菌酶，发现它们与细胞间空间内的阴离子成分呈时间相关反应。覆盖腹侧电细胞表面的基膜拥有最多的阴离子位点，而生长锥、突触前终末和神经胶质膜几乎没有染色。由于该基膜在突触形成过程中是向内生长的神经突的唯一底物，这些结果与以下观点一致，即膜表面的电荷分布可能为神经突的运动、延伸及最终的突触形成提供必要的线索。
