Marshak D W, Carraway R E, Ferris C F
Department of Neurobiology and Anatomy, University of Texas Medical School, Houston 77025.
Exp Eye Res. 1987 Jun;44(6):839-48. doi: 10.1016/s0014-4835(87)80046-5.
Extracts of goldfish retina and brain were analysed by high-pressure liquid chromatography and radioimmunoassays for neurotensin (NT), substance P (SP) and related peptides. Two major peaks of immunoreactive NT were observed, both more hydrophilic than mammalian NT. Although these two forms reacted equally well with two antisera towards the biologically active carboxyl terminal region of NT, they were not recognized by an amino terminal antiserum, nor by antisera toward the related peptides, LANT-6 and xenopsin. Retinal immunoreactive SP eluted as one major peak which may represent the teleost equivalent of mammalian SP; region-specific antisera indicated phylogenetic conservation of the carboxyl terminal region. A more hydrophilic immunoreactive form was found only in brain, and two more hydrophobic forms found in both tissues were relatively more abundant in retina than in brain. The concentrations and molecular forms of these peptides were unchanged after either 48 hr of light adaptation or 48 hr of dark adaptation.
采用高压液相色谱法和放射免疫分析法对金鱼视网膜和脑提取物中的神经降压素（NT）、P物质（SP）及相关肽进行了分析。观察到两个主要的免疫反应性NT峰，两者均比哺乳动物的NT更具亲水性。尽管这两种形式与针对NT生物活性羧基末端区域的两种抗血清反应同样良好，但它们不被氨基末端抗血清识别，也不被针对相关肽LANT - 6和异视蛋白的抗血清识别。视网膜免疫反应性SP洗脱为一个主要峰，这可能代表硬骨鱼中与哺乳动物SP相当的物质；区域特异性抗血清表明羧基末端区域具有系统发育保守性。仅在脑中发现一种更具亲水性的免疫反应形式，在两种组织中发现的另外两种更具疏水性的形式在视网膜中的含量相对比在脑中更丰富。在48小时的光适应或48小时的暗适应后，这些肽的浓度和分子形式均未改变。
