Holzer P, Bucsics A, Saria A, Lembeck F
Neuroscience. 1982;7(11):2919-24. doi: 10.1016/0306-4522(82)90114-2.
Immunoreactive substance P and neurotensin in extracts of the digestive tract of man, cat, guinea-pig, pig, rabbit, and rat were measured by radioimmunoassay using antisera directed against the C-terminal portions of the two peptides. In all species except the cat, the concentrations of substance P were highest in the small intestine, intermediate in the large intestine and lowest in the stomach and oesophagus: the digestive tract of the cat displayed a rather even distribution of substance P. As observed in the ileum of guinea-pig, rabbit, and rat, the external muscle layer including the myenteric plexus contained 2-5 times higher concentrations of substance P than the whole ileal wall, whereas the substance P concentrations in the mucosa were only about one sixth of those in the whole wall. High performance liquid chromatography of extracts of human, feline and rabbit ileum showed that all the immunoreactive substance P eluted at the positions of substance P and substance P sulfoxide. The distribution of immunoreactive neurotensin along the digestive system of all six species was very similar. The highest concentrations of neurotensin were measured in the distal part of the small intestine, whereas the large intestine, stomach and oesophagus contained only low concentrations of neurotensin relative to the concentrations in the ileum. As examined in the ileum of guinea-pig, rabbit, and rat, the mucosa exhibited 2.5-4 times higher concentrations of neurotensin than the whole ileal wall, while the concentrations of neutrotensin in the external muscle layer including the myenteric plexus were only 4-20% of those in the whole wall. High performance liquid chromatography of the immunoreactive neurotensin extracted from the cat ileum yielded a single peak corresponding to neurotensin while the immunoreactive neurotensin extracted from the ileum of man and rabbit was eluted in two peaks, 55 and 72% of the recovered immunoreactivity, respectively, corresponding to neurotensin. These findings are in line with the proposed roles of substance P in the neural, and neurotensin in the endocrine, control and maintenance of gastrointestinal motility.
使用针对这两种肽C末端部分的抗血清，通过放射免疫测定法测量了人、猫、豚鼠、猪、兔和大鼠消化道提取物中的免疫反应性P物质和神经降压素。除猫以外的所有物种中，P物质浓度在小肠中最高，在大肠中居中，在胃和食管中最低：猫的消化道中P物质分布较为均匀。如在豚鼠、兔和大鼠的回肠中观察到的，包括肌间神经丛的外肌层中P物质浓度比整个回肠壁高2至5倍，而黏膜中P物质浓度仅约为整个肠壁的六分之一。人、猫和兔回肠提取物的高效液相色谱显示，所有免疫反应性P物质在P物质和P物质亚砜的位置洗脱。所有六个物种的消化系统中免疫反应性神经降压素的分布非常相似。神经降压素浓度在小肠远端最高，而相对于回肠中的浓度，大肠、胃和食管中仅含有低浓度的神经降压素。如在豚鼠、兔和大鼠的回肠中检测到的，黏膜中神经降压素浓度比整个回肠壁高2.5至4倍，而包括肌间神经丛的外肌层中神经降压素浓度仅为整个肠壁的4%至20%。从猫回肠中提取的免疫反应性神经降压素的高效液相色谱产生一个对应于神经降压素的单峰，而从人及兔回肠中提取的免疫反应性神经降压素在两个峰中洗脱，分别占回收免疫反应性的55%和72%，对应于神经降压素。这些发现与P物质在神经方面以及神经降压素在内分泌方面对胃肠动力的控制和维持中所起的作用相符。
