Wang Y Z, Tavassoli M
Veterans Administration Medical Center, Jackson, MS 39216.
Exp Hematol. 1987 Oct;15(9):1001-3.
A method is described that permits easy morphological recognition of hemopoietic and stromal cells in the adherent layer of long-term marrow culture in light microscopy. The method relies on the solubilization of plasma membrane with Triton X-100 to permit differential staining of cytoskeleton in the two cell types.
本文描述了一种方法,该方法可在光学显微镜下轻松实现对长期骨髓培养贴壁层中造血细胞和基质细胞的形态学识别。该方法依靠用曲拉通X-100溶解质膜,以使两种细胞类型的细胞骨架实现鉴别染色。