Boswell H S, Niskanen E O, Coppola M A, Quesenberry P J
Exp Hematol. 1983 Apr;11(4):315-23.
Long-term liquid culture was used to assess the broad functional integrity of murine bone marrow after cryopreservation. Cryopreserved and thawed marrow contained not only renewable hematopoietic stem cells, but also stromal progenitor cells capable of transferring the hematopoietic microenvironment to liquid culture. Long-term liquid culture may be a superior method of assessing the functional potential of cryopreserved marrow when compared to stem cell assays alone.
长期液体培养用于评估冷冻保存后小鼠骨髓的广泛功能完整性。冷冻保存并解冻后的骨髓不仅含有可再生的造血干细胞，还含有能够将造血微环境转移至液体培养的基质祖细胞。与单独的干细胞检测相比，长期液体培养可能是评估冷冻保存骨髓功能潜力的一种更优方法。
