Masliah J, Kadiri C, Pepin D, Rybkine T, Etienne J, Chambaz J, Bereziat G
UA 524 CNRS, CHU Saint-Antoine, Paris, France.
FEBS Lett. 1987 Sep 28;222(1):11-6. doi: 10.1016/0014-5793(87)80182-5.
Polyclonal antiserum was prepared against phospholipase A2 from Naja naja and used to prepare a purified antibody. It cross-reacted with the antigen, and with intracellular mammalian PLA2. This antibody was immunoreactive and inhibited the PLA2 activity of Naja naja and of guinea pig alveolar macrophages or rat lymphocytes. By immunoblotting, this antiserum revealed one band of PLA2 from Naja naja (14 kDa) and 3 bands for guinea pig alveolar macrophages and rat lymphocytes (30, 45 kDa and a minor band of 14 kDa). These results show an antigenic relatedness between an extracellular PLA2 and membrane-bound PLA2 from two different mammalian species and cell types.
制备了针对眼镜蛇磷脂酶A2的多克隆抗血清，并用于制备纯化抗体。该抗体与抗原以及细胞内哺乳动物磷脂酶A2发生交叉反应。此抗体具有免疫反应性，可抑制眼镜蛇以及豚鼠肺泡巨噬细胞或大鼠淋巴细胞的磷脂酶A2活性。通过免疫印迹法，该抗血清显示出一条来自眼镜蛇的磷脂酶A2条带（14 kDa），以及豚鼠肺泡巨噬细胞和大鼠淋巴细胞的3条条带（30 kDa、45 kDa和一条14 kDa的次要条带）。这些结果表明，一种细胞外磷脂酶A2与来自两种不同哺乳动物物种和细胞类型的膜结合磷脂酶A2之间存在抗原相关性。
