Rudrammaji L M, Gowda T V
Department of Studies in Biochemistry, University of Mysore, Manasagangotri, India.
Toxicon. 1998 Apr;36(4):639-44. doi: 10.1016/s0041-0101(97)00096-2.
A PLA2-inhibitor has been purified from Indian cobra (Naja naja naja) venom by the combination of ion-exchange and gel-filtration chromatography. The inhibitor, NN-I3 was a peptide with mol.wt 6500 and has a fluorescence emission maxima ca 340 nm. NN-I3 specifically inhibited the enzyme activity of the three acidic PLA2 from the same venom. The inhibition of NN-I2d-PLA2 and NN-I2c-PLA2 by NN-I3 was of mixed type and NN-I2c-PLA2 was of uncompetitive type. Neither the inhibitor nor the individual mixtures of acidic PLA2 with the inhibitor (1:1 w/w or 1:2 mol:mol) were lethal to mice when injected intraperitoneally in doses up to 10 mg kg(-1) body weight.
通过离子交换和凝胶过滤色谱相结合的方法，从印度眼镜蛇（眼镜蛇指名亚种）毒液中纯化出一种磷脂酶A2抑制剂。该抑制剂NN-I3是一种分子量为6500的肽，其荧光发射最大值约为340nm。NN-I3特异性抑制来自同一种毒液的三种酸性磷脂酶A2的酶活性。NN-I3对NN-I2d-PLA2和NN-I2c-PLA2的抑制作用为混合型，对NN-I2c-PLA2的抑制作用为非竞争性。当以高达10mg kg(-1)体重的剂量腹腔注射时，抑制剂以及酸性磷脂酶A2与抑制剂的单独混合物（1:1 w/w或1:2 mol:mol）对小鼠均无致死性。
