Simon M, Hunyadi J
Department of Dermatology, University of Erlangen-Nürnberg, FRG.
Dermatologica. 1987;175(3):121-5. doi: 10.1159/000248809.
Antigenic characteristics of human keratinocytes (HK) were studied in positive intracutaneous tests for delayed-type hypersensitivity in vivo using murine monoclonal antibodies against human OKM5, OKM1, OKT6, Leu-7 and HLA-DR antigens and compared with those of negative skin reactions. By contrast to negative intracutaneous tests, keratinocytes displayed both OKM5 and HLA-DR antigens in all positive skin reactions above the dermal inflammatory infiltrate. OKM5 recognizes an 88-kilodalton cell surface determinant of monocytes, which could also be induced in vitro in keratinocytes upon incubation with gamma-interferon. We demonstrate here for the first time that the expression of this macrophage marker can also be induced in vivo in the HK.
利用抗人OKM5、OKM1、OKT6、Leu-7和HLA-DR抗原的鼠单克隆抗体，在体内阳性皮内试验中对迟发型超敏反应进行研究，以探讨人角质形成细胞（HK）的抗原特性，并与阴性皮肤反应的抗原特性进行比较。与阴性皮内试验不同，在真皮炎症浸润上方的所有阳性皮肤反应中，角质形成细胞均显示出OKM5和HLA-DR抗原。OKM5识别单核细胞的一种88千道尔顿的细胞表面决定簇，在用γ干扰素孵育后，角质形成细胞在体外也可诱导产生该决定簇。我们首次在此证明，这种巨噬细胞标志物的表达在体内HK中也可被诱导。
