Id1 对 TGF-β诱导的人真皮成纤维细胞胶原表达的抑制作用。
Inhibitory role of Id1 on TGF-β-induced collagen expression in human dermal fibroblasts.
机构信息
Department of Dermatology and Research Institute for Medical Sciences, School of Medicine, Chungnam National University, Daejeon, Republic of Korea.
Department of Dermatology and Research Institute for Medical Sciences, School of Medicine, Chungnam National University, Daejeon, Republic of Korea.
出版信息
Biochem Biophys Res Commun. 2014 Jan 31;444(1):81-5. doi: 10.1016/j.bbrc.2014.01.010. Epub 2014 Jan 14.
Inhibitor of DNA binding 1 (Id1) is a basic helix-loop-helix (bHLH) protein that has a variety of functional roles in cellular events including differentiation, cell cycle and cancer development. In addition, it has been demonstrated that Id1 is related with TGF-β and Smad signaling in various biological conditions. In this study, we investigated the effect of Id1 on TGF-β-induced collagen expression in human dermal fibroblasts. When Id1-b isoform was overexpressed, TGF-β-induced collagen expression was markedly inhibited. Consistent with this result, Id1-b significantly inhibited TGF-β-induced collagen gel contraction. In addition, Id1-b inhibited TGF-β-induced phosphorylation of Smad2 and Smad3. Finally, immunohistochemistry showed that Id1 expression was decreased in fibrotic skin diseases while TGF-β signaling was increased. Together, these results suggest that Id1 is an inhibitory regulator on TGF-β-induced collagen expression in dermal fibroblasts.
DNA 结合抑制因子 1（Id1）是一种碱性螺旋-环-螺旋（bHLH）蛋白，在包括分化、细胞周期和癌症发展在内的细胞事件中具有多种功能作用。此外，已经证明 Id1 在各种生物条件下与 TGF-β和 Smad 信号有关。在这项研究中，我们研究了 Id1 对人真皮成纤维细胞中 TGF-β 诱导的胶原蛋白表达的影响。当 Id1-b 同种型过表达时，TGF-β 诱导的胶原蛋白表达明显受到抑制。与该结果一致，Id1-b 显著抑制 TGF-β 诱导的胶原蛋白凝胶收缩。此外，Id1-b 抑制 TGF-β 诱导的 Smad2 和 Smad3 磷酸化。最后，免疫组织化学显示，在纤维化性皮肤病中 Id1 的表达减少，而 TGF-β 信号增加。总之，这些结果表明 Id1 是真皮成纤维细胞中 TGF-β 诱导的胶原蛋白表达的抑制调节因子。
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