Brogden Graham, Krimmling Tanja, Adamek Mikołaj, Naim Hassan Y, Steinhagen Dieter, von Köckritz-Blickwede Maren
Fish Disease Research Unit, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany; Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany.
Fish Disease Research Unit, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany; Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany; Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen University, PO Box 338, 6700 AH Wageningen, The Netherlands.
Dev Comp Immunol. 2014 Jun;44(2):280-5. doi: 10.1016/j.dci.2014.01.003. Epub 2014 Jan 14.
The formation of neutrophil extracellular traps (NETs) has been characterised as a novel antimicrobial host defence strategy of neutrophils besides phagocytosis and degranulation, which may lead to entrapment and subsequent immobilisation and/or killing of bacterial pathogens. Here we studied the effect of the feed additive β-glucan, namely MacroGard(®), on the formation and functionality of NETs in carp. Therefore, common carp (Cyprinus carpio) head kidney and kidney cells were isolated and treated with or without β-glucan over time. The formation of NETs was analysed by immunofluorescence microscopy and revealed a distinct increase of NET-formation with β-glucan. Furthermore the subsequent entrapment of Aeromonas hydrophila, an important fish pathogen, was increased after stimulating the cells with β-glucan. However, β-glucan did not lead to a stimulation of antimicrobial activity of neutrophils against A. hydrophila. In conclusion, the data underline the fact that the feed additive β-glucan is able to modulate carp neutrophil functions.
中性粒细胞胞外陷阱（NETs）的形成已被视为中性粒细胞除吞噬作用和脱颗粒作用之外的一种新型抗菌宿主防御策略，这可能导致细菌病原体的捕获以及随后的固定和/或杀灭。在此，我们研究了饲料添加剂β-葡聚糖（即MacroGard®）对鲤鱼NETs形成和功能的影响。因此，分离了鲤鱼（Cyprinus carpio）的头肾和肾细胞，并随时间用或不用β-葡聚糖进行处理。通过免疫荧光显微镜分析NETs的形成，结果显示β-葡聚糖使NETs的形成明显增加。此外，在用β-葡聚糖刺激细胞后，重要鱼类病原体嗜水气单胞菌的后续捕获增加。然而，β-葡聚糖并未导致中性粒细胞对嗜水气单胞菌的抗菌活性增强。总之，这些数据强调了饲料添加剂β-葡聚糖能够调节鲤鱼中性粒细胞功能这一事实。
