Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; Department of Physiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Life Sci. 2014 Mar 11;98(2):68-74. doi: 10.1016/j.lfs.2013.12.230. Epub 2014 Jan 13.
We aimed to evaluate the transcription and translation of genes for uncoupling protein 2 (UCP2) & uncoupling protein 3 (UCP3) in rat heart mitochondria of both ventricles after myocardial ischemia followed by various periods of reperfusion.
Seven groups of 8 male Wistar rats were evaluated for the effects of ischemia and also reperfusion, using Western blot of isolated mitochondrial proteins in addition to RNA extraction followed by real-time RT-PCR analysis.
In rats with 30 min of reperfusion (R30) UCP2 protein was increased 213±33%, which is meaningfully more than the control group (P<0.001). Western blot showed increase in UCP2 protein level in groups receiving reperfusion for 60 min (R60), 120 min (R120) and 180 min (R180) as much as 152±28% (P<0.001 vs. control), 123±19% (P<0.01 vs. control) and 131±30% (P<0.01 vs. control), respectively. There was no statistically important difference in UCP2 mRNA between either right or left ventricles of ischemic and ischemia-reperfusion (IR) groups vs. control group. In the groups R180 and R240, UCP3 protein levels showed 131±27% and 102±18% increase, respectively (both P<0.001 vs. control group). However, the change in UCP3 level in other groups was not significantly different from the control group.
UCP2 and UCP3 protein levels are considerably increased in the ischemic area early after acute myocardial IR. The right ventricular UCP2 protein expression does not change, that is, effect of IR on UCP2 protein is a local process. However, UCP3 protein level increased both in ischemic area of the left ventricle and in non-ischemic area of the right ventricle.
本研究旨在评估大鼠左右心室心肌缺血后不同再灌注时间点解偶联蛋白 2（UCP2）和 3（UCP3）的基因转录和翻译。
通过分离线粒体蛋白的 Western blot 及 RNA 提取和实时 RT-PCR 分析，评估 7 组雄性 Wistar 大鼠缺血及再灌注的影响，每组 8 只。
再灌注 30 分钟（R30）组 UCP2 蛋白增加 213±33%，明显高于对照组（P<0.001）。Western blot 显示，再灌注 60 分钟（R60）、120 分钟（R120）和 180 分钟（R180）组 UCP2 蛋白水平分别增加 152±28%（P<0.001 与对照组）、123±19%（P<0.01 与对照组）和 131±30%（P<0.01 与对照组）。缺血和缺血再灌注（IR）组左右心室的 UCP2 mRNA 与对照组相比均无统计学差异。在 R180 和 R240 组，UCP3 蛋白水平分别增加 131±27%和 102±18%（均 P<0.001 与对照组）。然而，其他组的 UCP3 水平变化与对照组无显著差异。
急性心肌 IR 后早期缺血区 UCP2 和 UCP3 蛋白水平显著增加。右心室 UCP2 蛋白表达不变，即 IR 对 UCP2 蛋白的影响是局部过程。然而，左心室缺血区和右心室非缺血区的 UCP3 蛋白水平均增加。
