Faculté des Sciences, Laboratoire de Biologie Moléculaire Végétale associé au CNRS L.A. 40), F-91405, Orsay, France.
Planta. 1975 Jan;125(2):171-80. doi: 10.1007/BF00388703.
The mtDNA (mitochondrial DNA) extracted from etiolated seedlings of Cucumis sativus L. has been purified by a three-step procedure: RNase and pronase treatment, bio-gel filtration, analytical CsCl gradient centrifugation. This procedure appeared rapid, suitable for small quantities of DNA and gave highly reproducible results. It was used to follow the quantitative variation of the mtDNA in hypocotyls and cotyledons of dark-grown cucumber seedlings.The major feature occurring during the etiolation process appeared to be an important accumulation of the mtDNA in hypocotyls between 3 and 5 days of culture. The amount of the mtDNA per hypocotyl increased 5 times, the hypocotyl length and the total DNA increased 6 and 12 times respectively, between these two stages. It was demonstrated that at least during the first week of culture in the dark, endogeneous hormone-induced elongation in the cucumber hypocotyl (a non-dividing tissue) was associated to an important accumulation of mtDNA.
从黄瓜（Cucumis sativus L.）黄化幼苗中提取的 mtDNA（线粒体 DNA）已通过三步程序进行了纯化：RNase 和蛋白酶处理、生物凝胶过滤、分析性 CsCl 梯度离心。该程序快速、适用于少量 DNA，并可获得高度可重复的结果。它用于跟踪黑暗培养的黄瓜幼苗下胚轴和子叶中 mtDNA 的定量变化。在黄化过程中出现的主要特征似乎是在培养的 3 到 5 天之间，下胚轴中 mtDNA 的大量积累。在下胚轴中，mtDNA 的含量增加了 5 倍，下胚轴的长度和总 DNA 分别增加了 6 倍和 12 倍。结果表明，至少在黑暗中培养的第一周内，内源激素诱导的黄瓜下胚轴（非分裂组织）伸长与 mtDNA 的大量积累有关。
