来自植物线粒体的DNA。
DNA from plant mitochondria.
作者信息
Suyama Y, Bonner W D
机构信息
Department of Biology, University of Pennsylvania.
出版信息
Plant Physiol. 1966 Mar;41(3):383-8. doi: 10.1104/pp.41.3.383.
DNA WAS ISOLATED FROM A MITOCHONDRIAL FRACTION OF EACH OF THE FOLLOWING PLANT MATERIALS
Mung bean (Phaseolus aureus) etiolated hypocotyl; turnip (Brassica rapa) root; sweet potato (Ipomoea batatas) root; and onion (Allium cepa) bulb. It was found that all of these mitochondrial fractions contained DNA, the densities of which were identical (rho=1.706 g.cm(-3)). An additional DNA (rho=1.695) band found in the mitochondrial fraction of Brassica rapa, was identical to DNA separately isolated from the chloroplast-rich fraction. The origin of the second DNA from Allium mitochondrial fraction was not identified.Contrary to the identity of the mitochondrial DNA, DNA from nuclear fractions differed not only with each other but from the corresponding mitochondrial DNA.DNA from Phaseolus and Brassica mitochondria showed the hyperchromicity characteristic of double stranded, native DNA upon heating; Tm's in 0.0195 Na(+) were the same; 72.0 degrees . The amount of DNA within the mitochondrion of Phaseolus was estimated to be 5.0 x 10(-10) mug; this estimate was made by isolating the mitochondrial DNA concomitantly with the known amount of added (15)N(2)H B. subtilis DNA (rho=1.740). Approximately the same amount of DNA was present in the mitochondrion of Brassica or Ipomoea.
从以下植物材料的线粒体部分分离出DNA：绿豆（Phaseolus aureus）黄化下胚轴；芜菁（Brassica rapa）根；甘薯（Ipomoea batatas）根；以及洋葱（Allium cepa）鳞茎。发现所有这些线粒体部分都含有DNA，其密度相同（ρ=1.706 g.cm(-3)）。在芜菁线粒体部分中发现的另一条DNA带（ρ=1.695）与从富含叶绿体的部分单独分离出的DNA相同。未确定来自洋葱线粒体部分的第二条DNA的来源。与线粒体DNA的一致性相反，来自核部分的DNA不仅彼此不同而且与相应的线粒体DNA也不同。来自菜豆和芜菁线粒体的DNA在加热时表现出双链天然DNA的增色特性；在0.0195 Na(+)中的熔解温度相同；为72.0度。菜豆线粒体中的DNA量估计为5.0×10(-10)微克；这个估计是通过将线粒体DNA与已知量的添加的（15）N(2)H枯草芽孢杆菌DNA（ρ=1.740）同时分离而得出的。芜菁或甘薯的线粒体中存在的DNA量大致相同。
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