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高等植物叶绿体脱氧核糖核酸和线粒体脱氧核糖核酸的动力学复杂性

Kinetic complexity of chloroplastal deoxyribonucleic acid and mitochondrial deoxyribonucleic acid from higher plants.

作者信息

Wells R, Birnstiel M

出版信息

Biochem J. 1969 May;112(5):777-86. doi: 10.1042/bj1120777.

DOI:10.1042/bj1120777
PMID:5821734
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1187784/
Abstract
  1. Chloroplasts and mitochondria were isolated by aqueous and non-aqueous cell-fractionation techniques. In a variety of higher plants the mitochondrial DNA bands in a caesium chloride gradient at 1.706g.cm.(-3), whereas chloroplastal DNA has a buoyant density of 1.697g.cm.(-3). 2. In total cellular DNA of moderate molecular weight, the chloroplastal DNA is found within the Gaussian distribution of the nuclear DNA and is not resolved as a satellite. 3. Both chloroplastal DNA and mitochondrial DNA from lettuce renature rapidly. 4. The kinetic complexity of mitochondrial DNA is > 10(8) daltons. 5. Chloroplastal DNA is made up from fast and slow renaturing sequences with kinetic complexities of 3x10(6) and 1.2x10(8) daltons respectively. 6. From the discrepancy between analytical and kinetic complexity it is concluded that chloroplastal DNA is extensively reiterated.
摘要
  1. 通过水相和非水相细胞分级分离技术分离叶绿体和线粒体。在多种高等植物中，线粒体DNA在氯化铯梯度中于1.706克/立方厘米处形成条带，而叶绿体DNA的浮力密度为1.697克/立方厘米。2. 在中等分子量的总细胞DNA中，叶绿体DNA处于核DNA的高斯分布范围内，未作为卫星DNA被分辨出来。3. 生菜的叶绿体DNA和线粒体DNA都能快速复性。4. 线粒体DNA的动力学复杂度大于10^8道尔顿。5. 叶绿体DNA由快速和慢速复性序列组成，其动力学复杂度分别为3×10^6和1.2×10^8道尔顿。6. 从分析复杂度和动力学复杂度之间的差异可以得出结论，叶绿体DNA有大量重复序列。
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fd5/1187784/73b3fc2d62ac/biochemj00702-0222-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fd5/1187784/73b3fc2d62ac/biochemj00702-0222-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fd5/1187784/73b3fc2d62ac/biochemj00702-0222-a.jpg

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