Dept. of Biochemistry, The University, S10 2TN, Sheffield, U.K..
Photosynth Res. 1986 Jan;10(3):297-302. doi: 10.1007/BF00118294.
Enhancement of photosynthesis by supplemental photosystem 1-enriched (707nm) light has been investigated in intact spinach chloroplasts by the simultaneous measurement of the rate of oxygen evolution, yield of chlorophyll fluorescence and quenching of 9-aminoacridine fluorescence. Chloroplasts reducing CO2 showed a 75% increase in the rate of O2 evolution after the addition of 707nm light, whereas if nitrite was used as substrate, an enhancement of only 20% was observed. Reduction of glycerate-3-phosphate was associated with a 40% enhancement by 707nm light. There appears to be a correlation between the degree of enhancement and the requirement for ATP in addition to reducing power. Prolonged illumination in 707nm light resulted in an elevation of enhancement whereas illumination with 650nm light caused a loss of enhancement, demonstrating the operation of state transitions in intact isolated chloroplasts.
通过同时测量氧的释放速率、叶绿素荧光的产量和 9-氨基吖啶荧光的猝灭，研究了用补充富含光系统 1 的（707nm）光增强完整菠菜叶绿体的光合作用。还原 CO2 的叶绿体在添加 707nm 光后，O2 释放速率增加了 75%，而如果使用亚硝酸盐作为底物，仅观察到 20%的增强。甘油-3-磷酸的还原与 707nm 光的 40%增强有关。增强的程度似乎与除了还原力之外还需要 ATP 的程度有关。在 707nm 光下长时间照射会导致增强升高，而用 650nm 光照射会导致增强丧失，这表明在完整的分离叶绿体中存在状态转变的操作。
