用非离子型去污剂和高浓度 NaCl 处理 PSII 膜以分离光系统 II 反应中心复合物的特性。
Characterization of a photosystem II reaction center complex isolated by exposure of PSII membranes to a non-ionic detergent and high concentrations of NaCl.
机构信息
Division of Biological Sciences, The University of Michigan, 48109-1048, Ann Arbor, MI, USA.
出版信息
Photosynth Res. 1986 Jan;10(3):483-8. doi: 10.1007/BF00118314.
A highly resolved PSII reaction center complex has been prepared by exposure of PSII membranes to the detergent octylglucopyranoside at elevated ionic strengths; oxygen evolution activity is about 1,000 μmoles O2/hr/mg Chl in the presence of CaCl2. A Mn quantitation and a kinetic study of Z, the donor to P680, reveals that on a Chl basis this new preparation shows an almost four-fold enrichment in Mn and the electron transport components of PSII.
已通过在高离子强度下用去污剂辛基葡萄糖苷处理 PSII 膜来制备高分辨率 PSII 反应中心复合物；在存在 CaCl2 的情况下，氧释放活性约为每毫克叶绿素 1000 μmoles O2/hr。对 Z（P680 的供体）的 Mn 定量和动力学研究表明，就 Chl 而言，这种新制备方法使 PSII 的 Mn 和电子传递成分富集了近四倍。
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