Chlorophyll fluorescence transients from the diatom Phaeodactylum tricornutum: relative rates of cyclic phosphorylation and chlororespiration.

In Phaeodactylum tricornutum cells kept 30 min in the dark, induction of fluorescence showed the well-known levels OIDPSMT. The decrease of MT was the most important when the intensity of excitation light was high. It was mainly due to the photochemical quenching. After addition of DCMU (2 to 20 μM), a quenching qE was still observed: this quenching, cancelled by NH4Cl (2 to 20 mM) is attributed to ΔpH. This qE was also inhibited by antimycin, an inhibitor of cyclic phosphorylation and may be of chlororespiration above plastoquinones. Anaerobiosis also decreased it. We can infer that chlororespiration also plays a part in the formation of the ΔpH in the presence of DCMU. After 30 mn of preillumination in red light, the levels P and M were lower and the quenching in presence of DCMU was no more observed: thus, neither the chlororespiration nor the cyclic phosphorylation were active, unless the activity of ATPase was much more important. So, in diatoms, one at least of the above cited phenomena can be modulated by light.