Department of Chemistry, Rensselaer Polytechnic Institute, 12180-3590, Troy, NY, USA.
Photosynth Res. 1987 Jan;12(2):145-54. doi: 10.1007/BF00047944.
Inhibition of photosystem 2 by the peptide-modification reagent, tetranitromethane, has been investigated with spinach digitonin particles. In the presence of tetranitromethane, (1) the initial fluoresence yield is suppressed with a concomitant elimination of the variable component of fluorescence; (2) the optical absorption transient at 820 nm, attributed to P680(+), is greatly attenuated; (3) diphenylcarbazide-supported photoreduction of dichlorophenol indophenol is abolished; and (4) electron spin resonance Signal 2f and Signal 2s are eliminated. These results are consistent with multiple sites of modification in photosystem 2 by tetranitromethane, and suggest further that this reagent can inhibit charge stabilization in the reaction center.
用菠菜的胆堿皂素颗粒研究了肽修饰试剂四硝甲烷对光系统 2 的抑制作用。在四硝甲烷存在的情况下：(1) 初始荧光产率受到抑制，同时消除了荧光的可变量；(2) 归因于 P680(+) 的 820nm 光吸收瞬变大大减弱；(3) 二氯酚靛酚的二苯基卡巴肼支持的光还原被废除；(4) 电子自旋共振信号 2f 和信号 2s 被消除。这些结果与四硝甲烷在光系统 2 中多部位修饰一致，并进一步表明该试剂可以抑制反应中心的电荷稳定化。
