Geysen H M, Rodda S J, Mason T J, Tribbick G, Schoofs P G
Department of Molecular Immunology, Commonwealth Serum Laboratories, Parkville, Victoria, Australia.
J Immunol Methods. 1987 Sep 24;102(2):259-74. doi: 10.1016/0022-1759(87)90085-8.
A recently developed approach to the synthesis and ELISA screening of large numbers of peptides is described. The method has created the opportunity to tackle questions about the sites and specificity of antigenic determinants which were formerly thought to be too difficult to answer. The various strategies for application of this method are described along with examples of their successful use. They include a procedure for locating all the continuous antigenic peptides of a protein antigen, and the identification of non-replaceable amino acid residues within an antigenic peptide. An approach to the determination of amino acid residues involved in the epitope for any monoclonal antibody is also described. These strategies open up the prospect of rapid mapping of the antigenic properties of hitherto poorly understood antigens.
本文描述了一种最近开发的用于合成和ELISA筛选大量肽段的方法。该方法为解决有关抗原决定簇的位点和特异性问题创造了机会，而这些问题以前被认为太难回答。文中描述了该方法的各种应用策略及其成功应用的实例。这些策略包括确定蛋白质抗原所有连续抗原肽段的程序，以及鉴定抗原肽段内不可替代的氨基酸残基。还描述了一种确定任何单克隆抗体表位中涉及的氨基酸残基的方法。这些策略为快速绘制迄今了解甚少的抗原的抗原特性开辟了前景。
