Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo, 13083-865, Brazil.
Muscle Nerve. 2014 Aug;50(2):283-6. doi: 10.1002/mus.24177. Epub 2014 Jun 17.
The purpose of this study was to better understand the beneficial effects of doxycycline on the dystrophic muscles of the mdx mouse.
Doxycycline (DOX) was administered for 36 days, starting on postnatal day 0, via drinking water. Untreated mdx mice received plain water for the same period and served as a control group.
DOX decreased the levels of metalloproteinase-9 and tumor necrosis factor-alpha in the biceps brachii and diaphragm of the mdx mice. It also reduced the total amount of calcium in the muscles studied, concomitant with an increase in the levels of calsequestrin 1.
The results show that DOX can affect factors that are important in dystrophic pathogenesis and highlight its potential as a readily accessible therapy in clinical trials for treatment of Duchenne muscular dystrophy.
本研究旨在更好地理解强力霉素对 mdx 小鼠肌肉营养不良的有益作用。
强力霉素（DOX）通过饮用水从出生后第 0 天开始给药 36 天。未治疗的 mdx 小鼠在同一时期接受普通水，作为对照组。
DOX 降低了 mdx 小鼠肱二头肌和膈肌中基质金属蛋白酶-9 和肿瘤坏死因子-α的水平。它还减少了研究肌肉中的总钙量，同时增加了钙结合蛋白 1 的水平。
结果表明 DOX 可以影响肌肉营养不良发病机制中的重要因素，并突出其作为临床试验中治疗杜氏肌营养不良症的一种易于获得的治疗方法的潜力。
