Murphy T F, Bernstein J M, Dryja D M, Campagnari A A, Apicella M A
Department of Medicine, State University of New York at Buffalo.
J Infect Dis. 1987 Nov;156(5):723-31. doi: 10.1093/infdis/156.5.723.
We studied isolates of nontypable Haemophilus influenzae from cultures of nasopharynx and middle ear fluid (MEF) done simultaneously on children with otitis media. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of outer membrane protein (OMP) patterns demonstrated that in 16 of 19 pairs, the nasopharyngeal and MEF strains were identical. With four monoclonal antibodies to lipooligosaccharide (LOS) determinants, 17 of the 19 pairs were identical. Thus the pathogenesis of otitis media due to nontypable H. influenzae appears to involve spread of the bacteria from the nasopharynx to the middle ear. Analysis of middle ear isolates from four children with recurrent otitis media caused by nontypable H. influenzae indicated that the recurrent episodes were caused by reinfection with different strains rather than by persistence of the same strain. OMP and LOS analysis of strains from two sisters with concurrent otitis media suggested that person-to-person transmission of nontypable H. influenzae can occur among children.
我们研究了从患有中耳炎的儿童同时采集的鼻咽部和中耳积液（MEF）培养物中分离出的不可分型流感嗜血杆菌。外膜蛋白（OMP）模式的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳表明，在19对样本中的16对中，鼻咽部和MEF菌株是相同的。使用四种针对脂寡糖（LOS）决定簇的单克隆抗体，19对样本中的17对是相同的。因此，不可分型流感嗜血杆菌引起的中耳炎发病机制似乎涉及细菌从鼻咽部传播到中耳。对四名由不可分型流感嗜血杆菌引起复发性中耳炎的儿童的中耳分离株分析表明，复发是由不同菌株的再次感染引起的，而不是由同一菌株的持续存在引起的。对两名并发中耳炎的姐妹的菌株进行OMP和LOS分析表明，不可分型流感嗜血杆菌在儿童之间可发生人传人传播。
