Department of Biological Sciences, Purdue University, 47907, Lafayette, Indiana, USA.
Planta. 1975 Jan;122(1):67-78. doi: 10.1007/BF00385406.
Changes in the structure and composition of cell walls of Fucus gardneri Silva were related to embryo development. Results of histochemical treatments of walls from embryos of different ages were compared with those of differential extractions and electronmicroscopic examinations of isolated walls. At 24 h after fertilization alginic acid, fucoidin and cellulose were structural constituents of the embryo wall. The distribution of alginic acid and cellulose was uniform over the cell walls of embryos of any age, but fucoidin was concentrated at the rhizoid end of cell walls isolated from germinated embryos. Evidence is presented for the presence of sulfated xylogalactofucoglucuronan and β,1-3 glucan in the embryo cell-wall. Electron micrographs of untreated and differentially extracted cell walls showed that alginic acid comprised the fibrillar material and fucoidin comprised the amorphous material of the wall. Fucus eggs do not have cell walls and the zygotes began wall synthesis within 40 min after fertilization. During the first 2 h there was a rapid deposition of alginic acid. After 4 h the wall thickness increased linearly through 24 h. Embryos treated with cycloheximide ceased wall growth after 6 h. Wall isolated from 24-h-old cycloheximide-treated embryos resembled wall isolated from control embryos 2-4 h old.
藻体结构和组成的变化与石莼胚胎发育有关。比较了不同年龄胚胎细胞壁的组织化学处理结果与差异提取和分离细胞壁的电子显微镜检查结果。受精后 24 小时，藻酸、褐藻糖胶和纤维素是胚胎细胞壁的结构成分。在任何年龄的胚胎细胞壁上，藻酸和纤维素的分布都是均匀的，但褐藻糖胶集中在从发芽胚胎中分离出的细胞壁的根状末端。有证据表明，在胚胎细胞壁中有硫酸化木糖半乳醛酸阿拉伯半乳聚糖和β，1-3 葡聚糖。未经处理和差异提取的细胞壁的电子显微镜照片显示，藻酸组成了纤维状物质，褐藻糖胶组成了细胞壁的无定形物质。石莼卵没有细胞壁，受精卵在受精后 40 分钟内开始合成细胞壁。在最初的 2 小时内，藻酸迅速沉积。4 小时后，细胞壁厚度通过 24 小时线性增加。用环己亚胺处理的胚胎在 6 小时后停止细胞壁生长。从 24 小时龄环己亚胺处理的胚胎中分离出的细胞壁与 2-4 小时龄的对照胚胎中分离出的细胞壁相似。
