Long Ashton Research Station, University of Bristol, BS18 9AF, Bristol, U.K..
Planta. 1975 Jan;122(2):203-7. doi: 10.1007/BF00388659.
A number of cytokinin reference compounds have been successfully separated by High Pressure Liquid Chromatography using columns of pellicular strong cation exchange resin and of pellicular polyamide. On polyamide, all cytokinins were eluted within 10 min with an aqueous buffer but on the cation exchange resin some cytokinins (generally those with bulky N(6)-substituents and in addition lacking an N(9)-ribosyl substituent) had excessively long retention times with aqueous buffer eluent. However, addition of methanol to the buffer enabled these cytokinins to be separated and eluted within a reasonable time. As small an amount as 5 nanogram of cytokinin could readily be detected by the procedures described.
已成功使用颗粒状强阳离子交换树脂和颗粒状聚酰胺柱通过高压液相色谱法将几种细胞分裂素参考化合物分离。在聚酰胺上，所有细胞分裂素都在 10 分钟内用水性缓冲液洗脱，但在阳离子交换树脂上，一些细胞分裂素（通常是那些具有大体积 N(6)-取代基并且另外缺乏 N(9)-核糖基取代基的细胞分裂素）用水性缓冲液洗脱时保留时间过长。然而，向缓冲液中添加甲醇可使这些细胞分裂素在合理的时间内分离和洗脱。通过所述方法可容易地检测到低至 5 纳克的细胞分裂素。
