Takei Kentaro, Yamaya Tomoyuki, Sakakibara Hitoshi
Plant Science Center, RIKEN (The Institute of Physical and Chemical Research), 1-7-22 Suehiro, Tsurumi, Yokohama 230-0045, Japan.
J Plant Res. 2003 Jun;116(3):265-9. doi: 10.1007/s10265-003-0099-1. Epub 2003 May 7.
Recent progress in the study of cytokinin metabolism in plants indicates that quantitative analysis of cytokinin nucleotides is essential for elucidation of early steps of the biosynthetic pathway. However, traditional procedures for purification and quantification of cytokinin cannot discriminate the various nucleotides. We describe here a method for separation and determination of cytokinin nucleotides through a series of anion-exchange column chromatography steps followed by liquid chromatography-mass spectrometry. This method enabled us to analyze the amount of each species of cytokinin nucleotide in plant tissues.
植物细胞分裂素代谢研究的最新进展表明，对细胞分裂素核苷酸进行定量分析对于阐明生物合成途径的早期步骤至关重要。然而，传统的细胞分裂素纯化和定量方法无法区分各种核苷酸。我们在此描述一种通过一系列阴离子交换柱色谱步骤，随后进行液相色谱 - 质谱联用的细胞分裂素核苷酸分离和测定方法。该方法使我们能够分析植物组织中每种细胞分裂素核苷酸的含量。
