Brodie C, Sampson S R
Department of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
J Pharmacol Exp Ther. 1987 Sep;242(3):1104-8.
Effects of chronic ethanol treatment were examined on transmembrane resting potential (Em), electrogenic pump component of Em and on Na-K pump activity of cultured rat skeletal myotubes. Properties of Na-K pump activity were determined by measurement of [3H]ouabain binding and 86Rb uptake by myotubes after incubation of myotubes in ethanol (217 mM) for up to 9 days. Chronic ethanol exposure caused an increase in Em, an increase in electrogenic pump component of Em and a decrease in depolarizing response to addition of ethanol to the myotubes. Chronic ethanol also produced increases in Na-K pump sites and in Na-K pump activity. Thus, chronic ethanol treatment causes a compensatory increase in amount and activity of the Na-K pump, and this results in an increased expression of the electrogenic pump component of Em. These effects may represent the membrane expression of tolerance to the depressant effects of ethanol.
研究了慢性乙醇处理对培养的大鼠骨骼肌管跨膜静息电位（Em）、Em的电生泵成分以及Na-K泵活性的影响。通过在乙醇（217 mM）中孵育肌管长达9天，然后测量肌管对[3H]哇巴因的结合和86Rb的摄取，来确定Na-K泵活性的特性。慢性乙醇暴露导致Em升高、Em的电生泵成分增加以及向肌管中添加乙醇后去极化反应减弱。慢性乙醇还使Na-K泵位点和Na-K泵活性增加。因此，慢性乙醇处理导致Na-K泵的数量和活性代偿性增加，这导致Em的电生泵成分表达增加。这些效应可能代表了对乙醇抑制作用耐受性的膜表达。
