非洲大蜗牛神经元中A电流通道的选择性和膜片钳测量
Selectivity and patch measurements of A-current channels in Helix aspersa neurones.
作者信息
Taylor P S
机构信息
Department of Physiology and Biophysics, University of Washington, Seattle 98195.
出版信息
J Physiol. 1987 Jul;388:437-47. doi: 10.1113/jphysiol.1987.sp016623.
Abstract
- The ionic selectivity of A-current K+ channels has been measured in single Helix aspersa neurones by recording the reversal potential shift in test solutions containing various monovalent cations. 2. The A-current channel is permeable to Tl+, K+, Rb+, NH4+ and Cs+. The channels may also be sparingly permeable to Na+ and Li+. Organic cations have an apparent small permeability as judged from their reversal potentials, but this may be an artifact of K+ accumulation. 3. A large patch electrode (3 microns tip) isolated a region that appeared to contain only A-current channels. This may indicate that A-current channels are found in the membrane as rafts of at least 3 microns in diameter. 4. The single-channel conductance calculated from single-channel current steps was 14 pS.
摘要
- 通过记录含有各种单价阳离子的测试溶液中的反转电位偏移，已在单个蛞蝓神经元中测量了A电流钾通道的离子选择性。2. A电流通道对铊离子、钾离子、铷离子、铵根离子和铯离子具有通透性。这些通道对钠离子和锂离子也可能有少量通透性。从它们的反转电位判断，有机阳离子的通透性明显较小，但这可能是钾离子积累的假象。3. 一个大的膜片电极（尖端3微米）隔离出一个似乎只包含A电流通道的区域。这可能表明A电流通道以直径至少3微米的筏状形式存在于膜中。4. 根据单通道电流阶跃计算出的单通道电导为14皮西门子。
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