Pang Lei, Zhang Yuerong, Liu Zhonglin
Dept. of Oral Anatomy and Physiology, College of Stomatology, Zunyi Medical College, Zunyi 563003, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2013 Dec;31(6):628-32.
To determine the effects of different estrogen concentrations on the proliferation and differentiation of human embryo mandibular condylar chondrocytes (MCCs).
In vitro-cultured MCCs were incubated and treated with estrogen at different concentrations. The MCCs were identified by toluidine blue and type II collagen immunocytochemical methods, and cell proliferation was monitored using methyl thiazolyl tetrazolium assay.
The addition of 10(-12) or 10(-6) mol x L(-1) exogenous estrogen inhibited the growth of MCCs, whereas addition of 10(-10) or 10(-8) mol x L(-1) exogenous estrogen promoted growth. These estrogen effects were time-dependent.
Estrogen can affect cellular MCCs proliferation and differentiation in a biphasic manner depending on the concentration. Estrogen may be vital in the maintenance of normal functions of the temporomandibular joint (TMJ) and may be involved in the progression of certain TMJ diseases.
确定不同雌激素浓度对人胚胎下颌髁突软骨细胞（MCCs）增殖和分化的影响。
将体外培养的MCCs用不同浓度的雌激素进行孵育和处理。通过甲苯胺蓝和II型胶原免疫细胞化学方法鉴定MCCs，并使用甲基噻唑基四氮唑法监测细胞增殖。
添加10⁻¹²或10⁻⁶ mol·L⁻¹外源性雌激素抑制MCCs生长，而添加10⁻¹⁰或10⁻⁸ mol·L⁻¹外源性雌激素促进生长。这些雌激素作用具有时间依赖性。
雌激素可根据浓度以双相方式影响MCCs细胞的增殖和分化。雌激素可能对颞下颌关节（TMJ）正常功能的维持至关重要，并且可能参与某些TMJ疾病的进展。
