Department of Stomatology, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, PR China.
Department of Stomatology, Shenzhen Sixth People's Hospital (Nanshan Hospital) Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, PR China.
Arch Oral Biol. 2020 Jan;109:104570. doi: 10.1016/j.archoralbio.2019.104570. Epub 2019 Sep 23.
The aim of this study is to investigate the effects of 17β-Estradiol (E2) at different concentrations combined with cyclical compressive stress on the proliferation and differentiation of mandibular condylar chondrocytes (MCCs).
MCCs, isolated from female Sprague-Dawley rats, were exposed to E2 at different concentration, cyclical compressive stress or the combination, effects of which on MCCs proliferation and differentiation were detected.
E2 at physiological concentration (10 mol/L) has lower proliferative effects on MCCs, compared with non-physiological concentration (10 mol/L or 10 mol/L). For MCCs differentiation, effects of E2 at different concentration are totally opposite: E2 at 10 mol/L promotes MCCs differentiation, but at 10 mol/L or 10 mol/L, it inhibits MCCs differentiation. When combined with E2 at 10 mol/L, cyclical compressive stress shows synergistic effect on proliferation and differentiation. However, when combined with E2 at 10 mol/L or 10 mol/L cyclical compressive stress reverses the inhibition in MCCs differentiation provoked by E2 at 10 mol/L or 10 mol/L.
Effects of E2 combined with cyclical compressive stress on MCCs proliferation and differentiation are different, which suggests that orthodontist should take fully consideration of the levels of E2 and adopt comprehensive strategies, so as to achieve better orthodontic effect.
本研究旨在探讨不同浓度 17β-雌二醇（E2）联合周期性压应力对下颌髁突软骨细胞（MCCs）增殖和分化的影响。
从雌性 Sprague-Dawley 大鼠中分离出 MCCs，分别用不同浓度的 E2、周期性压应力或两者联合作用，检测其对 MCCs 增殖和分化的影响。
生理浓度（10 毫摩尔/升）的 E2 对 MCCs 的增殖作用低于非生理浓度（10 毫摩尔/升或 10 毫摩尔/升）。对于 MCCs 分化，不同浓度的 E2 作用完全相反：10 毫摩尔/升的 E2 促进 MCCs 分化，但 10 毫摩尔/升或 10 毫摩尔/升的 E2 则抑制 MCCs 分化。当与 10 毫摩尔/升的 E2 联合使用时，周期性压应力对增殖和分化具有协同作用。然而，当与 10 毫摩尔/升或 10 毫摩尔/升的 E2 联合使用时，周期性压应力会逆转 10 毫摩尔/升的 E2 对 MCCs 分化的抑制作用。
E2 联合周期性压应力对 MCCs 增殖和分化的影响不同，这提示正畸医生应充分考虑 E2 的水平，并采取综合策略，以达到更好的正畸效果。
