Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo , 33006 Oviedo, Spain.
J Agric Food Chem. 2014 Feb 19;62(7):1733-40. doi: 10.1021/jf500118x. Epub 2014 Feb 10.
A reliable and robust direct screening methodology based on a quantum dot (QD) fluorescent immunoassay has been developed to detect trace levels of different antibiotic species from the family of the tetracyclines (e.g., oxytetracycline, tetracycline, chlortetracycline, and doxycycline) in contaminated bovine muscle tissues. First, the synthesis and characterization of a new immunoprobe (oxytetracycline-bovine serum albumin-QD) has been carried out for its further application in the development of a competitive fluorescent QD-immunoassay. The developed fluoroimmunoassay provides sensitive and binary "yes/no" responses being appropriate for the screening of this family of antibiotics above or below a preset concentration threshold. The detection limit achieved with this strategy, 1 μg/L in aqueous media and 10 μg/kg in bovine muscle samples, is 10-fold lower than the maximum level concentration allowed by International Legislation in muscle tissue, enabling suitable and efficient screening of the antibiotics.
已经开发出一种可靠且稳健的基于量子点（QD）荧光免疫测定的直接筛选方法，用于检测来自四环素族（例如，土霉素、四环素、金霉素和强力霉素）的不同抗生素种类的痕量水平在污染的牛肌肉组织中。首先，已经进行了新免疫探针（土霉素-牛血清白蛋白-QD）的合成和表征，以便将其进一步应用于竞争性荧光 QD-免疫测定的开发中。所开发的荧光免疫测定法提供了敏感的二元“是/否”响应，适用于在预设浓度阈值以上或以下筛选此类抗生素。通过该策略实现的检测限，在水介质中为 1μg/L，在牛肌肉样品中为 10μg/kg，比肌肉组织中国际法规允许的最大允许浓度低 10 倍，从而能够对抗生素进行适当且有效的筛选。
