Duan Xiaoke, Zhang Xiujie, Song Wen, Wang Yizhou, Gao Zexia, Wang Weimin
a Key Lab of Freshwater Animal Breeding , College of Fisheries, Ministry of Agriculture, Huazhong Agricultural University , Wuhan , Hubei , People's Republic of China and.
b Freshwater Aquaculture Collaborative Innovation Center of Hubei Province , Wuhan , Hubei , People's Republic of China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):86-7. doi: 10.3109/19401736.2013.873915. Epub 2014 Jan 17.
Parabramis pekinensis strenosoma belongs to the family Cyprinidae. In the present study, we obtain the complete mitochondrial genome of P. pekinensis strenosoma by PCR amplification and DNA sequencing. It is a circular double-stranded DNA molecule of 16,623 base pairs in length, consisting of the typical structure of 22 transfer RNA genes, 13 protein-coding genes, 2 ribosomal RNA genes, as well as 2 main non-coding regions (the control region and the origin of the light strand replication). Compared with Parabramis pekinensis, the two different subspecies share 99.58% nucleotide sequence similarity and the biggest nucleotide sequence discrepancy between homologous genes are observed in ND2 for protein-coding genes and in tRNA-Ala for tRNA-coding genes. The complete mitochondrial genome sequence data are of great use for phylogenetic analysis and studies of population genetics and germplasm resources of P. pekinensis strenosoma.
尖头鲌属于鲤科。在本研究中，我们通过PCR扩增和DNA测序获得了尖头鲌的完整线粒体基因组。它是一个长度为16,623个碱基对的环状双链DNA分子，由22个转移RNA基因、13个蛋白质编码基因、2个核糖体RNA基因以及2个主要非编码区（控制区和轻链复制起点）的典型结构组成。与翘嘴鲌相比，这两个不同亚种的核苷酸序列相似度为99.58%，在蛋白质编码基因中，同源基因之间最大的核苷酸序列差异出现在ND2基因；在tRNA编码基因中，最大差异出现在tRNA-Ala基因。完整的线粒体基因组序列数据对于尖头鲌的系统发育分析以及种群遗传学和种质资源研究具有重要意义。
