Zhu Xinpeng, Ma Zhihong, Yang Xuefen, Xu Hongxia, Yang Ruibin
a Key Laboratory of Freshwater Animal Breeding certificated by Ministry of Agriculture , College of Fisheries, Huazhong Agricultural University , Wuhan , China and.
b Freshwater Aquaculture Collaborative Innovation Center of Hubei Province , Wuhan , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):589-90. doi: 10.3109/19401736.2014.908358. Epub 2014 Apr 14.
Acheilognathus macropterus belongs to the family Acheilognathinae. In the present study, we obtain the complete mitochondrial genome of the Chinese bitterling A. macropterus by PCR amplification and DNA sequencing. It is a circular double-stranded DNA molecule of 16,773 bp in length, containing 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and two main non-coding regions (the control region and the origin of the light strand replication). The gene composition and order of which are similar to most other vertebrates. Compared with Acanthorhodeus macropterus, they share 98.90% nucleotide sequences similarity, and the biggest nucleotide sequence discrepancy between homologous genes are observed in ND5 gene. The molecular data we presented here could provide useful information for the studies on species identification, evolutionary relationships and population genetics of the Acheilognathinae.
大鳍鱊属于鱊亚科。在本研究中，我们通过PCR扩增和DNA测序获得了中华鳑鲏大鳍鱊的完整线粒体基因组。它是一个长度为16773 bp的环状双链DNA分子，包含13个蛋白质编码基因、22个转运RNA基因、两个核糖体RNA基因以及两个主要的非编码区（控制区和轻链复制起始点）。其基因组成和顺序与大多数其他脊椎动物相似。与大鳍刺鳑鲏相比，它们的核苷酸序列相似度为98.90%，同源基因之间最大的核苷酸序列差异出现在ND5基因中。我们在此提供的分子数据可为鱊亚科的物种鉴定、进化关系和群体遗传学研究提供有用信息。
