Mu Xi-Dong, Liu Chao, Wang Xue-Jie, Liu Yi, Hu Yin-Chang, Luo Jian-Ren
a Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation , Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Ministry of Agriculture , Guangzhou , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):44-5. doi: 10.3109/19401736.2013.869687. Epub 2014 Jan 17.
We determined the complete mitochondrial genome of Tilapia buttikoferi, which was 16,577 bp in length with an A + T content of 53.0%, containing 13 protein-coding genes, 2 rRNAs, 22 tRNAs and a complete control region. The gene arrangement was similar to that of typical fishes. The total base composition of the mitogenome was 25.6% T, 30.8% C, 27.4% A and 16.2% G. Of the 13 protein-coding genes, 12 genes start with an ATG codon, except for COX1 with GTG. Seven (ND1, ND2, COX1, ATPase8, ATPase6, ND4L and ND6) used TAA or AGA as the termination codon, whereas six (COX2, COX3, ND3, ND4, ND5 and cyt b) had incomplete stop codon T. Its control region was atypical in being short at 861 bp, and contained TACAT motif and one microsatellite-like region (TA)7. This mitogenome sequence data may be useful for phylogenetic and systematic analyses within the family Cichlaidae.
我们测定了布氏罗非鱼的完整线粒体基因组，其长度为16,577 bp，A + T含量为53.0%，包含13个蛋白质编码基因、2个rRNA、22个tRNA和一个完整的控制区。基因排列与典型鱼类相似。线粒体基因组的总碱基组成为25.6%的T、30.8%的C、27.4%的A和16.2%的G。在13个蛋白质编码基因中，12个基因以ATG密码子起始，除了COX1以GTG起始。7个基因（ND1、ND2、COX1、ATPase8、ATPase6、ND4L和ND6）使用TAA或AGA作为终止密码子，而6个基因（COX2、COX3、ND3、ND4、ND5和细胞色素b）具有不完整的终止密码子T。其控制区不典型，较短，为861 bp，包含TACAT基序和一个微卫星样区域(TA)7。该线粒体基因组序列数据可能对丽鱼科内的系统发育和系统分析有用。
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