Jang Yo-Soon, Park Kwang-Jae, Kim Ki-Yong, Kim Sung
a East Sea Environment Research Division , Korea Institute of Ocean Science & Technology , Gyeongbuk , Republic of Korea .
b Department of Marine Biotechnology , University of Science & Technology , Daejeon , Republic of Korea .
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):69-70. doi: 10.3109/19401736.2013.873904. Epub 2014 Jan 17.
Due to variations in coloration as well as other morphological features, the identification of Sebastes pachycephalus has been problematic. The complete mitogenome of S. pachycephalus was acquired using next-generation sequencing. The full genome was 16,415 base pairs (bp) in length, assembling two rRNAs, 22 tRNAs, one control region and 13 protein-coding genes (PCGs). The genome constitutes 27.9% A, 26.3% T, 17.2% G and 28.5% C, showing a slight AT bias (54.2%). All PCGs start with an ATG initial codon except COX1, which contains GTG. Most PCG stop codons were TAA, except for ND1 and ND3 (TAG) and Cytb (incomplete termination codon, T). All tRNAs showed the typical cloverleaf structure, except tRNA(Ser(AGY)), which lacks the DHU arm. The complete mitogenome of S. pachycephalus will help further identification and speciation analyses.
由于颜色以及其他形态特征的差异，厚头平鲉的鉴定一直存在问题。利用二代测序技术获得了厚头平鲉的完整线粒体基因组。该全基因组长度为16,415个碱基对（bp），组装得到两个rRNA、22个tRNA、一个控制区和13个蛋白质编码基因（PCG）。基因组中A占27.9%，T占26.3%，G占17.2%，C占28.5%，显示出轻微的AT偏向性（54.2%）。除COX1以GTG作为起始密码子外，所有PCG均以ATG作为起始密码子。大多数PCG的终止密码子为TAA，除了ND1和ND3为TAG，Cytb为不完全终止密码子T。除了缺少DHU臂的tRNA(Ser(AGY))外，所有tRNA均呈现典型的三叶草结构。厚头平鲉的完整线粒体基因组将有助于进一步的鉴定和物种形成分析。
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