[使用高效排阻色谱法测定微生物多糖的分子量]
[Determination of the molecular weight of microbial polysaccharides using high performance exclusion chromatography].
作者信息
Chlenov M A, Iarovaia S M, Alekseeva G S, Kudriashov L I, Titova E V
出版信息
Prikl Biokhim Mikrobiol. 1987 Jul-Aug;23(4):515-21.
An automatic method of determining the molecular weight parameters (Mw, Mn) of microbial polysaccharides such as dextran, pullulan was developed based on the use of high performance size-exclusion chromatography on the two types of columns: Zorbax PSM 60 + 300 + 1000 and SynChropack GPC 100 + 500 + 1000. The Mw and Mn values were determined for a number of domestic and foreign dextran preparations. Changes in the molecular weight of pullulan and hydroxyethylstarch resulted from acid and enzymatic hydrolysis were estimated.
基于在两种类型的色谱柱（Zorbax PSM 60 + 300 + 1000和SynChropack GPC 100 + 500 + 1000）上使用高效尺寸排阻色谱法，开发了一种自动测定微生物多糖（如葡聚糖、支链淀粉）分子量参数（Mw、Mn）的方法。测定了许多国内外葡聚糖制剂的Mw和Mn值。评估了酸和酶水解导致的支链淀粉和羟乙基淀粉分子量的变化。
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