Wallace M A, Salley S O, Barnhart M I
Department of Medical Technology, College of Pharmacy and Allied Health Professions Wayne State University, Detroit, Michigan 48202.
Scanning Microsc. 1987 Sep;1(3):1387-95.
Transmission electron microscopy (TEM) was used to determine the effect of diesel engine exhaust (DEE) on the intravascular and interstitial cellular population of the lungs of exposed rats and guinea pigs. Animals with matched controls were subjected to environments of either 250, 750, 1500 or 6000 micrograms/m3 for either 2 weeks, 6 weeks, 10 weeks or 18 months. These animals were sacrificed immediately following the exposure periods and their lungs perfused with fixative. Following dissection, random stratified biopsies from the lungs of these animals were made. Ultrathin sections from the alveolar lung were prepared and conventionally processed for TEM and randomly photographed to compose a micrograph database. These micrographs were analyzed by point counting using a Zeiss MOP 3 Digital Image Analyzer. The results indicated no significant intravascular cellular response but a significant increase in the mononuclear population in the interstitium.
采用透射电子显微镜（TEM）来确定柴油机尾气（DEE）对暴露的大鼠和豚鼠肺部血管内及间质细胞群体的影响。将匹配的对照动物置于250、750、1500或6000微克/立方米的环境中，暴露时间分别为2周、6周、10周或18个月。暴露期结束后立即处死这些动物，并向其肺部灌注固定剂。解剖后，从这些动物的肺部进行随机分层活检。制备来自肺泡肺的超薄切片，并进行常规处理以用于TEM，然后随机拍照以构建显微照片数据库。使用蔡司MOP 3数字图像分析仪通过点计数对这些显微照片进行分析。结果表明血管内细胞无明显反应，但间质中的单核细胞群体显著增加。
