Warren H B, Pantazis P, Davies P F
Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts 02115.
Am J Pathol. 1987 Oct;129(1):9-13.
Monotypic cultures of passaged human umbilical vein endothelial cells secrete C3 protein, which is specifically immunoprecipitable from cell lysates and conditioned medium. A transcript migrating at 5.3 kilobases hybridizes with a mouse-derived C3 DNA probe in parallel cultures of endothelial cells, hepatoma cells (HepG2), and freshly isolated human monocytes. Steady-state transcript levels were as follows: HepG2 cells, 1.0; monocytes, 0.05; endothelial cells, 0.01. These data suggest that C3, an indispensible component for activation of complement by both the classical and alternative pathways, is locally synthesized in the vascular bed.
传代培养的人脐静脉内皮细胞的单型培养物分泌C3蛋白，该蛋白可从细胞裂解物和条件培养基中特异性免疫沉淀。在内皮细胞、肝癌细胞（HepG2）和平行培养的新鲜分离的人单核细胞中，一条迁移率为5.3千碱基的转录本与小鼠来源的C3 DNA探针杂交。稳态转录水平如下：HepG2细胞为1.0；单核细胞为0.05；内皮细胞为0.01。这些数据表明，C3是经典途径和替代途径激活补体的必需成分，在血管床中局部合成。
