Juneja R K, Gahne B, Stratil A
Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala.
Anim Genet. 1987;18(2):119-24. doi: 10.1111/j.1365-2052.1987.tb00750.x.
Pig, horse and dog plasma proteins, separated by horizontal polyacrylamide gel electrophoresis (pH 9.0) and electrophoretically transferred to nitrocellulose membranes, were tested for cross-reaction with antiserum to human plasma alpha 1B-glycoprotein (alpha 1B). The results showed that one previously reported polymorphic plasma postalbumin in each of these species (pig PO2, horse Xk and dog Pa protein) was homologous to human plasma alpha 1B. In the light of the previously known genetic linkages in these species, this implied: (1) alpha 1B gene is close linked to Phi, Pgd and Hal (halothane sensitivity locus) loci in pigs; and (2) alpha 1B gene is linked to ME1 and Phi loci in horses. This suggested that the alpha 1B gene may also be found to be closely linked to gene(s) controlling susceptibility to malignant hyperthermia in humans and other mammals.
通过水平聚丙烯酰胺凝胶电泳（pH 9.0）分离猪、马和犬的血浆蛋白，并将其电泳转移至硝酸纤维素膜上，检测其与抗人血浆α1B-糖蛋白（α1B）抗血清的交叉反应。结果显示，这些物种中各自先前报道的一种多态性血浆后白蛋白（猪的PO2、马的Xk和犬的Pa蛋白）与人血浆α1B同源。鉴于这些物种中先前已知的遗传连锁关系，这意味着：（1）α1B基因在猪中与Phi、Pgd和Hal（氟烷敏感性位点）位点紧密连锁；（2）α1B基因在马中与ME1和Phi位点连锁。这表明α1B基因在人类和其他哺乳动物中可能也被发现与控制恶性高热易感性的基因紧密连锁。
