猪的血清蛋白PO2、马的Xk和人的α1B-糖蛋白之间的同源性。

The homology between the serum proteins PO2 in pig, Xk in horse and alpha 1B-glycoprotein in human.

Van de Weghe A, Coppieters W, Bauw G, Vandekerckhove J, Bouquet Y

Department of Animal Genetics, State University of Ghent, Merelbeke, Belgium.

Comp Biochem Physiol B. 1988;90(4):751-6. doi: 10.1016/0305-0491(88)90330-6.

Abstract

Pig serum Po2 protein and horse Xk protein were purified by FPLC, non-denaturing 2D agarose-PAGE and 2D IPG-PAGE. 2. The separated fractions were electroblotted to poly(4-vinyl-N-methylpyridinium iodide) coated GF/C glass fiber sheets. 3. The partial amino acid sequences and amino acid compositions of different genetic variants of the proteins were determined. 4. The results proved that previously reported polymorphic serum post-albumins in each of these species were homologous to human plasma alpha 1B-glycoprotein.

摘要

通过快速蛋白质液相色谱法（FPLC）、非变性二维琼脂糖凝胶电泳和二维固相pH梯度凝胶电泳（2D IPG-PAGE）对猪血清Po2蛋白和马Xk蛋白进行纯化。2. 将分离的组分电印迹到涂有聚（4-乙烯基-N-甲基碘化吡啶）的GF/C玻璃纤维片上。3. 测定了这些蛋白质不同遗传变体的部分氨基酸序列和氨基酸组成。4. 结果证明，此前报道的这些物种中多态性血清后白蛋白与人血浆α1B-糖蛋白同源。