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耐铜十足目动物维吉尼亚螯虾(哈根)(螯虾科,甲壳纲)的血淋巴特征

Haemolymph characteristics of a copper-tolerant decapod Orconectes virilis (Hagen) (Astacidae, Crustacea).

作者信息

Naich M, Alikhan M A

机构信息

Department of Biology, Laurentian University, Sudbury, Ontario, Canada.

出版信息

Arch Int Physiol Biochim. 1987 Jun;95(2):133-9. doi: 10.3109/13813458709104525.

DOI:10.3109/13813458709104525
PMID:2444180
Abstract

The mean haemolymph volume in a copper-tolerant, intermoult (C2-C4) adult (more than a year old) Orconectes virilis, as determined by [14C-carboxylic] inulin dilution method, ranged from 61.17 microliters in females (mean wet body weight = 4.34 g; mean carapace length = 4.43 cm) to 61.79 microliters in males (mean wet weight = 5.73 g; mean carapace length = 4.49 cm). The mean haemolymph volume per cent varied from 14.5 (in females) to 31.1 (in males). Haemolymph from males had an average pH of 7.35, osmotic pressure of 414.1 mmol/kg H2O, and a specific gravity of 1.0603, while that from females averaged a pH of 7.48, osmotic pressure of 433.6 mmol/kg H2O and a specific gravity of 1.0469. Mean total haemocyte counts ranged from 3,434/mm3 (1,000/mm3 halinocytes, 1,125/mm3 hemi-granulocytes and 1,309/mm3 granulocytes) in males to 4,905/mm3 (1,525/mm3 halinocytes. 1,490/mm3 hemi-granulocytes and 1,890/mm3 granulocytes) in females.

摘要

采用[¹⁴C - 羧酸]菊粉稀释法测定,耐铜的成年(一岁以上)雄性和雌性中间蜕壳期(C2 - C4)维吉尼亚螯虾的平均血淋巴体积范围如下:雌性为61.17微升(平均湿体重 = 4.34克;平均头胸甲长度 = 4.43厘米),雄性为61.79微升(平均湿体重 = 5.73克;平均头胸甲长度 = 4.49厘米)。血淋巴体积百分比均值在雌性中为14.5%,在雄性中为31.1%。雄性血淋巴的平均pH值为7.35,渗透压为414.1 mmol/kg H₂O,比重为1.0603;而雌性血淋巴的平均pH值为7.48,渗透压为433.6 mmol/kg H₂O,比重为1.0469。血细胞总数均值范围为:雄性为3434/mm³(1000/mm³ 卤细胞、1125/mm³ 半颗粒细胞和1309/mm³ 颗粒细胞),雌性为4905/mm³(1525/mm³ 卤细胞、1490/mm³ 半颗粒细胞和1890/mm³ 颗粒细胞)。

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