Zhang Yu Shrike, Yao Junjie, Wang Lihong V, Xia Younan
The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA.
Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130, USA.
Polymer (Guildf). 2014 Jan 14;55(1):445-452. doi: 10.1016/j.polymer.2013.06.019.
Cell patches are widely used for healing injuries on the surfaces or interfaces of tissues such as those of epidermis and myocardium. Here we report a novel type of porous scaffolds made of poly(D,L-lactic--glycolic acid) for fabricating cell patches. The scaffolds have a single layer of spherical pores arranged in a unique hexagonal pattern and are therefore referred to as "scaffolds with a hexagonal array of interconnected pores (SHAIPs)". SHAIPs contain both uniform pores and interconnecting windows that can facilitate the exchange of biomacromolecules, ensure homogeneous cell seeding, and promote cell migration. As a proof-of-concept demonstration, we have created skeletal muscle patches with a thickness of approximately 150 μm using SHAIPs. The myoblasts seeded in the scaffolds maintained high viability and were able to differentiate into multi-nucleated myotubes. Moreover, neovasculature could efficiently develop into the patches upon subcutaneous implantation .
细胞片广泛用于修复诸如表皮和心肌等组织表面或界面的损伤。在此，我们报告一种由聚（D，L-乳酸-乙醇酸）制成的新型多孔支架，用于制造细胞片。这些支架具有单层呈独特六边形排列的球形孔，因此被称为“具有相互连接孔的六边形阵列支架（SHAIPs）”。SHAIPs包含均匀的孔和相互连接的窗口，可促进生物大分子的交换，确保细胞均匀接种，并促进细胞迁移。作为概念验证演示，我们使用SHAIPs制备了厚度约为150μm的骨骼肌片。接种在支架中的成肌细胞保持高活力，并能够分化为多核肌管。此外，皮下植入后，新生血管能够有效地在片中形成。
