使用多焦平面显微镜的3D单分子追踪与超分辨率显微镜技术
3D single molecule tracking and superresolution microscopy using multifocal plane microscopy.
作者信息
Ram Sripad, Ward E Sally, Ober Raimund J
机构信息
Department of Immunology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Department of Immunology, University of Texas Southwestern Medical Center, Dallas, TX, USA; Department of Electrical Engineering, University of Texas at Dallas, Richardson, TX, USA.
出版信息
Proc IEEE Int Symp Biomed Imaging. 2012 Dec 31;2012:914-915. doi: 10.1109/ISBI.2012.6235702.
The study of cellular processes in three-dimensions is severely limited by the lack of imaging methodologies that allow for fast 3D tracking of cellular events and 3D superresolution imaging of sub-cellular structures. We have developed a 3D imaging modality, multifocal plane microscopy (MUM), that provides a powerful approach for 3D single molecule tracking and 3D superresolution microscopy. Here we review the technical challenges associated with 3D single molecule localization that is fundamental to both 3D tracking and 3D superresolution, and discuss how MUM overcomes these problems.
三维细胞过程的研究受到成像方法的严重限制，这些方法无法实现细胞事件的快速三维追踪以及亚细胞结构的三维超分辨率成像。我们开发了一种三维成像模式，即多焦平面显微镜（MUM），它为三维单分子追踪和三维超分辨率显微镜提供了一种强大的方法。在此，我们回顾了与三维单分子定位相关的技术挑战，这对于三维追踪和三维超分辨率都至关重要，并讨论了MUM如何克服这些问题。
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