Belisle E H, Su S W, Lubit B W, Fu S C
Department of Biochemistry, UMDNJ-New Jersey Medical School, Newark 07103.
Curr Eye Res. 1987 Aug;6(8):951-7. doi: 10.3109/02713688709034866.
The mouse monoclonal antibodies, prepared with rat beta H-crystallin as parent antigen, show cross-reactivity to specific subunits in beta H-, beta L1-, and beta L2-crystallin but no reactivity to either alpha- or gamma-crystallins. Antigenic homology among the beta-crystallin subunits was demonstrated by comparison of the topographic distributions of peptides on two-dimensional electrophoretograms subjected to either staining or an ELISA of the immunoblots.
以大鼠βH-晶状体蛋白作为亲本抗原制备的小鼠单克隆抗体，对βH-、βL1-和βL2-晶状体蛋白中的特定亚基具有交叉反应性，但对α-或γ-晶状体蛋白均无反应性。通过对二维电泳图谱上经染色或免疫印迹ELISA处理的肽段的拓扑分布进行比较，证实了β-晶状体蛋白亚基之间的抗原同源性。
