Carper D, Smith-Gill S J, Kinoshita J H
Curr Eye Res. 1984 Mar;3(3):501-7. doi: 10.3109/02713688408997238.
A monoclonal antibody to a bovine lens 27K beta-crystallin polypeptide has been produced from a rat x mouse hybridoma. The antibody reacts with the 27K polypeptide of bovine, mouse, rat, and human lenses, but does not react to the 27K polypeptide of monkey lens nor does it react with any component in the Philly mouse lens which is missing the 27K polypeptide. The antibody does not recognize any of the other major bovine beta-crystallin polypeptides but does recognize a large number of native beta-crystallin proteins. The antigenic specificity and species cross-reactivity of this antibody provides an excellent opportunity to study many aspects of lens development and cataractogenesis.
一种针对牛晶状体27Kβ-晶状体蛋白多肽的单克隆抗体已由大鼠与小鼠的杂交瘤产生。该抗体能与牛、小鼠、大鼠和人晶状体的27K多肽发生反应，但不与猴晶状体的27K多肽发生反应，也不与缺失27K多肽的费城小鼠晶状体中的任何成分发生反应。该抗体不识别任何其他主要的牛β-晶状体蛋白多肽，但能识别大量天然的β-晶状体蛋白。这种抗体的抗原特异性和种间交叉反应性为研究晶状体发育和白内障形成的许多方面提供了绝佳机会。
