The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21287, USA.
Christian Medical College Vellore, Tamil Nadu 632004, India.
Hum Pathol. 2014 Apr;45(4):880-3. doi: 10.1016/j.humpath.2013.10.016. Epub 2013 Oct 31.
Digital polymerase chain reaction is a new technology that enables detection and quantification of cancer DNA molecules from peripheral blood. Using this technique, we identified mutant PIK3CA DNA in circulating ptDNA (plasma tumor DNA) from a patient with concurrent early stage breast cancer and non-small cell lung cancer. The patient underwent successful resection of both her breast and lung cancers, and using standard Sanger sequencing the breast cancer was shown to harbor the identical PIK3CA mutation identified in peripheral blood. This case report highlights potential applications and concerns that can arise with the use of ptDNA in clinical oncology practice.
数字聚合酶链反应是一种新技术，能够从外周血中检测和定量癌症 DNA 分子。使用该技术，我们在一位同时患有早期乳腺癌和非小细胞肺癌的患者的循环 ptDNA（血浆肿瘤 DNA）中鉴定出突变的 PIK3CA DNA。该患者成功地接受了乳腺癌和肺癌的切除术，并且使用标准的 Sanger 测序显示乳腺癌具有在外周血中鉴定出的相同的 PIK3CA 突变。本病例报告强调了在临床肿瘤学实践中使用 ptDNA 可能出现的潜在应用和问题。
