优化提取、分离和定量分析西兰花叶中十二种完整的硫代葡萄糖苷。
Optimized extraction, separation and quantification of twelve intact glucosinolates in broccoli leaves.
机构信息
IU CINQUIMA, Analytical Chemistry Group, University of Valladolid, 47011 Valladolid, Spain.
IU CINQUIMA, Analytical Chemistry Group, University of Valladolid, 47011 Valladolid, Spain.
出版信息
Food Chem. 2014;152:66-74. doi: 10.1016/j.foodchem.2013.11.125. Epub 2013 Nov 28.
A new method has been developed and validated to determine twelve intact glucosinolates (glucoiberin, GIB; glucoraphanin, GRA; glucoerucin GER; gluconapin, GNA; glucotropaeolin, GTL; glucobrassicin, GBC; gluconasturtiin, GST; glucoalyssin, ALY; 4-hydroxyglucobrassicin, 4-OH; 4-metoxyglucobrassicin, 4ME; neoglucobrassicin, NEO; sinigrin, SIN) in broccoli leaves using liquid chromatography (LC) coupled to diode array (DAD) and electrospray ionization mass spectrometry (ESI-MS) detection. An extraction procedure has also been proposed and optimized by means of statistical analysis (the Box-Behnken design and analysis of variance); this is based on the deactivation of myrosinase using a microwave and heated water. Low limits of detection and quantification were obtained, ranging from 10 to 72 μg/g with DAD and 0.01 to 0.23 μg/g with ESI-MS, and the resulting recovery values ranged from 87% to 106% in all cases. Finally, glucosinolates were analyzed in broccoli leaf samples from six different cultivars (Ramoso calabrese Parthenon, Marathon, Nubia, Naxos and Viola).
已开发并验证了一种新方法，用于测定十二种完整的硫代葡萄糖苷（葡萄糖苷，GIB；萝卜硫素，GRA；葡萄糖异硫氰酸酯，GER；葡萄糖苷，GNA；葡萄糖芸苔素，GTL；硫代葡萄糖苷，GBC；葡萄糖芥子油苷，GST；丙硫氨酸芥子油苷，ALY；4-羟基硫代葡萄糖苷，4-OH；4-甲氧基硫代葡萄糖苷，4ME；新葡萄糖苷，NEO；黑芥子硫苷酸钾，SIN）在西兰花叶中的含量，采用液相色谱（LC）与二极管阵列（DAD）和电喷雾电离质谱（ESI-MS）检测相结合的方法。还通过统计分析（Box-Behnken 设计和方差分析）提出并优化了一种提取程序；该程序基于使用微波和热水使黑芥子酶失活。使用 DAD 可获得 10 至 72 μg/g 的低检测限和定量限，使用 ESI-MS 可获得 0.01 至 0.23 μg/g 的低检测限和定量限，所有情况下的回收率值均在 87%至 106%之间。最后，对来自六个不同品种（Ramoso calabrese Parthenon、Marathon、Nubia、Naxos 和 Viola）的西兰花叶样品中的硫代葡萄糖苷进行了分析。
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