Department of Analytical Chemistry, Institute of Chemistry, University of Bialystok, Hurtowa 1, 15-399 Bialystok, Poland.
Department of Analytical Chemistry, Institute of Chemistry, University of Bialystok, Hurtowa 1, 15-399 Bialystok, Poland.
Food Chem. 2014;152:155-61. doi: 10.1016/j.foodchem.2013.11.138. Epub 2013 Nov 28.
It was found that weak chemiluminescence of manganese(IV)-hexametaphosphate-formaldehyde system was greatly enhanced by plant polyphenolic compounds. Based on this finding, a new flow injection chemiluminescence method (FI-CL) was developed for the determination of the total content of polyphenols in plant extracts. The calibration graph obtained for standard solutions of 6-hydroxyluteolin 7-O-glucoside (6OHLG) was linear in the range 0.001-0.8 μg mL(-1). The method was simple, rapid (203 samples h(-1)) and sensitive with a detection limit of 0.25 ng mL(-1). The FI-CL method was successfully applied to the determination of the total polyphenols content (as 6OHLG equivalents) in methanolic, ethyl acetate and aqueous extracts from leaves of Cirsium palustre (L.). Two types of solvent extraction methods (reflux and ultrasound assisted extraction) were used and compared in terms of extraction efficiency. A positive, significant linear correlation between the results obtained by FI-CL method and spectrophotometric methods was observed.
研究发现植物多酚化合物能显著增强高锰酸根-六偏磷酸-甲醛体系的弱化学发光。基于这一发现，建立了一种新的流动注射化学发光法（FI-CL），用于测定植物提取物中总多酚的含量。以 6-羟基木犀草素 7-O-葡萄糖苷（6OHLG）标准溶液获得的校准曲线在 0.001-0.8μg mL（-1）范围内呈线性。该方法简单、快速（203 个样品 h（-1）），灵敏度高，检测限为 0.25ng mL（-1）。FI-CL 法成功应用于测定柳兰（Cirsium palustre（L.））叶甲醇、乙酸乙酯和水提取物中总多酚（以 6OHLG 当量计）的含量。比较了回流和超声辅助提取两种溶剂提取方法的提取效率。FI-CL 法与分光光度法的测定结果之间存在显著的正线性相关关系。
Molecules. 2023-10-19
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