Department of Medical Microbiology, Faculty of Medicine, Tekirdag Namik Kemal University, 59030 Tekirdag, Turkey.
Department of Food Technology, Vocational School of Technical Sciences, Tekirdag Namik Kemal University, 59030 Tekirdag, Turkey.
Molecules. 2023 Oct 19;28(20):7177. doi: 10.3390/molecules28207177.
The aim of this study was to investigate the compounds in the hexane extract of (Savi.) Ten. and to determine the antibacterial, antifungal, and antioxidant activities of different extracts. The (NGBB 7229) plant was collected from Turkey's Trakya region. Crude extracts were obtained using different solvents. The chemical composition of was determined in hexane extract using gas chromatography mass spectrometry. The antioxidant activities of the extracts were evaluated by Trolox equivalent antioxidant activity (TEAC), ferric-reducing antioxidant power (FRAP), cupric-reducing antioxidant capacity (CUPRAC), the β-carotene bleaching method, and the determination of superoxide anion scavenging activities. The antibacterial activity was tested against , , , , , and , whereas the antifungal activity was tested against , , and by applying microdilution methods. A total of 41 bioactive compounds were identified using the GC-MS library. Terpenoids were found to be dominant (52.89%), and lup-20(29)-en-3-yl-acetate and lupeol were the most abundant terpenoids. The highest total flavonoid content (25.73 mg catechin/g) and antioxidant capacity were found in the methanolic extract. The highest antibacterial activity was detected against in the ethyl acetate extract, and the highest antifungal activity was found against and in the hexane extract. The observed antioxidant characteristics of the extracts could be attributed to the presence of flavonoids. The high antifungal activity of the hexane extract against all fungal strains can be attributed to its constituents, i.e., terpenoids. This study discloses the potential antioxidant and antimicrobial activities, including some bioactive components, of and implies that holds possible applications in the food and pharmaceutical industries as an antioxidant, antibacterial, and antifungal agent.
本研究旨在研究 （Savi.）Ten.的正己烷提取物中的化合物，并测定不同提取物的抗菌、抗真菌和抗氧化活性。该植物（NGBB 7229）来自土耳其的特拉基亚地区。使用不同溶剂获得粗提取物。使用气相色谱质谱联用仪确定正己烷提取物中的化学成分。通过 Trolox 当量抗氧化活性 (TEAC)、铁还原抗氧化能力 (FRAP)、铜还原抗氧化能力 (CUPRAC)、β-胡萝卜素漂白法和超氧阴离子清除活性评估提取物的抗氧化活性。采用微量稀释法测试提取物对 、 、 、 、 和 的抗菌活性，测试对 、 、 、 和 的抗真菌活性。使用 GC-MS 库鉴定了 41 种具有生物活性的化合物。萜类化合物占主导地位（52.89%），其中最丰富的萜类化合物为 lup-20(29)-en-3-yl-acetate 和 lupeol。在甲醇提取物中发现总类黄酮含量最高（25.73 mg 儿茶素/g）和抗氧化能力最强。在乙酸乙酯提取物中检测到最强的抗菌活性，对 ，在正己烷提取物中检测到最强的抗真菌活性，对 和 。观察到的 提取物的抗氧化特性可能归因于类黄酮的存在。正己烷提取物对所有真菌菌株的高抗真菌活性可归因于其成分，即萜类化合物。本研究揭示了 潜在的抗氧化和抗菌活性，包括一些生物活性成分，并表明 作为抗氧化剂、抗菌剂和抗真菌剂在食品和制药行业具有潜在的应用。
