Department of Analytical Chemistry, Institute of Chemistry, University of Białystok, Białystok, Poland.
Department of Pharmacognosy, Medical University of Białystok, Białystok, Poland.
J Sep Sci. 2017 Oct;40(19):3830-3838. doi: 10.1002/jssc.201700496. Epub 2017 Aug 30.
Novel methods for the determination of polyphenolic antioxidants present in extracts from inflorescences of Cirsium vulgare (Savi) Ten. based on ultra-high performance liquid chromatography with photodiode array and chemiluminescence detection have been developed. Under the optimized conditions of chromatographic separation the analytical characteristic of the method was performed. The proposed method was successfully applied to the determination of ten polyphenols present in inflorescences of Cirsium vulgare. A comparison of the contents of analytes in extracts prepared by using various extraction media (methanol, ethanol, 70% methanol, 70% ethanol, and water) was carried out for the first time. For the postcolumn detection of scavenging activity of polyphenolic antioxidants against reactive oxygen species (H O , OH, O ) three systems based on chemiluminescence of luminol were used. A review of the current scientific literature shows that this is the first report on the application of luminol-based postcolumn detection for the on-line investigation of OH scavenging activity. The main compound determined in extracts from inflorescences of Cirsium vulgare was apigenin 7-O-glucuronide, whereas the highest antioxidant activity was observed for chlorogenic acid, luteolin 7-O-glucoside, and apigenin.
已经开发出了基于超高效液相色谱-光电二极管阵列和化学发光检测法，用于测定来自普通蓟（Savi）总状花序提取物中多酚类抗氧化剂的新方法。在色谱分离的优化条件下，对该方法的分析特性进行了评价。该方法成功地应用于测定普通蓟总状花序中十种多酚类化合物的含量。首次比较了用不同提取介质（甲醇、乙醇、70%甲醇、70%乙醇和水）制备的提取物中分析物的含量。为了对多酚类抗氧化剂清除活性氧（H2O2、OH、O2-）的柱后检测，使用了基于鲁米诺化学发光的三个系统。对当前科学文献的回顾表明，这是首次报道基于鲁米诺的柱后检测在在线研究 OH 清除活性中的应用。在普通蓟总状花序提取物中测定的主要化合物是芹黄素 7-O-葡萄糖醛酸苷，而绿原酸、木樨草素 7-O-葡萄糖苷和芹黄素表现出最高的抗氧化活性。
