Forest Genetics and Eco-physiology Research Group, School of Forest Engineering, Technical University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain.
Forest Genetics and Eco-physiology Research Group, School of Forest Engineering, Technical University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain.
Plant Physiol Biochem. 2014 Mar;76:1-6. doi: 10.1016/j.plaphy.2013.12.023. Epub 2014 Jan 8.
The presence and characteristics of cuticle polysaccharides have been demonstrated by staining and spectroscopic methods, but their location in the cuticle remains unclear. Furthermore, according to the prevailing model, polysaccharides are believed to be restricted to the cuticular layer and absent in the cuticle proper. With the aim of gaining insight into cuticular ultra-structure focussing on polysaccharides, cellulose and pectins have been identified and located in the transversal sections of isolated and intact adaxial leaf cuticles of Eucalyptus globulus, Populus × canescens and Pyrus communis by means of enzyme gold-labelling (Au-cellulase, EC 3.2.1.4, and -pectinase, EC 3.2.1.15) and transmission electron microscopy (TEM). The structure of the interface between the cuticle and the cell wall underneath was observed to influence the process of enzymatic isolation of leaf cuticles. Cellulose and pectins were detected for the first time in enzymatically isolated cuticles, sometimes appearing closely underneath the epicuticular wax layer. The location and presence of polysaccharides in intact and isolated leaf cuticles may have multiple implications, such as when estimating the bi-directional transport of substances between plant organs and the surrounding environment, or when interpreting organ ontogeny. The results are discussed within a plant ontological and ecophysiological context.
通过染色和光谱方法已经证明了表皮多糖的存在和特征，但它们在表皮中的位置仍不清楚。此外，根据流行的模型，多糖被认为仅限于表皮层，而不在表皮本身中。为了深入了解表皮的超微结构，特别是多糖，已经通过酶金标记（Au-纤维素酶，EC 3.2.1.4 和 -果胶酶，EC 3.2.1.15）和透射电子显微镜（TEM）在分离和完整的叶表皮的横切面上鉴定和定位了桉树、杨属和梨属的表皮中的纤维素和果胶。观察到表皮和细胞壁之间的界面结构会影响叶片表皮的酶分离过程。纤维素和果胶首次在酶分离的表皮中被检测到，有时出现在表皮蜡层的下方。完整和分离的叶片表皮中多糖的位置和存在可能具有多种意义，例如在估计植物器官与周围环境之间的物质双向运输时，或者在解释器官发生时。结果在植物发生学和生态生理学的背景下进行了讨论。
